Complete nucleotide sequence of full length cDNA for rat α cardiac myosin hea chain
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چکیده
منابع مشابه
Heart myosin light chain 2 gene. Nucleotide sequence of full length cDNA and expression in normal and hypertensive rat.
We have isolated and characterized a cDNA recombinant plasmid (pRLC429) specific for the rat heart myosin light chain 2 (MLC2). The cDNA insert consists of 446 base pairs, including a 72-base pair segment of the 3'-untranslated region. Additional 5'-sequence, not present in plasmid pRLC429, was obtained by primer extension of the cDNA. The extended cDNA sequence combined with the plasmid pRLC42...
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Pyruvate kinase (PK), a cytosolic enzyme, catalyzes the conversion of phosphoenolpyruvate' to pyruvate with the generation of ATP. We have isolated a full length cDNA clone from a Xgtll cDNA library constructed from rat pituitary GH3 cells by using a 475 bp Pstl fragment from a human PK cDNA (1). The sequence is 2310 bp long and contains a long open reading frame of 1593 bp with an initiation c...
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A cDNA (1.6 kilobases) for branched chain acyltransferase (E2b) isolated from a human liver library encoded only the amino-terminal half of the protein (Hummel, K. B., Litwer, S., Bradford, A. P., Aitken, A., Danner, D. J., and Yeaman, S. J. (1988) J. Biol. Chem. 263, 6165-6168). Here we report the isolation of other cDNAs which encode the carboxyl-terminal half of E2b and the construction of a...
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We have cloned in EScherichia coli a cDNA copy of mRNA coding for the porcine antral mucosal hormone preprogastrin. Full-length double-stranded cDNA was synthesized and inserted into the Pst I endonuclease site in plasmid pBR322 by using a homopolymeric extension technique. A partial cDNA clone was used as a probe to identify a complete cDNA clone in a cDNA library by colony hybridization. Four...
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A full-length cDNA clone encoding rabbit liver arylamine *• N-acetyltransferase (NAT; EC 2.3.1.5) was isolated from a Xgtll [ expression library by concomitant screening with an oligonucleof tide deduced from partial amino acid sequences (1) and with antibodies raised against the purified protein. The nucleotide p sequence of the clone, designated rnat, consists of 1102 nucleotides and contains...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.18.7527